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Proceedings Paper

Sensor enhanced microfluidic devices for cell based assays and organs on chip
Author(s): Claudia Gärtner; Birgit Ungerböck; Ingo Schulz; Tobias Jahn; Alexander Mosig; Torsten Mayr; Holger Becker
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Paper Abstract

Cell-based assays and organ-like substrates gather increasing attention due to their potentials in diagnostic and drug development. The use of these cell-based systems will allow to better understand in-vivo processes and to test for the direct influence of different substances on cell viability or metabolic activity e.g. in drug development and in addition to identify the influence of generated metabolites or different cell types. In this paper we present a respective technical platform, which enables the use of such cell-based assays. The platform is based on a microfluidic cell-assay toolbox, designed in a fashion allowing to minimize manual steps for cell culture on chip. Elements being essential for this work include membrane elements integrated into a microfluidic device for the separation of liquid stream together with a targeted supply of reagents and a three dimensional feeding of embedded cells. The influence of the metabolism from one cell type on the other can be evaluated due to the arrangement of cell compartments as interacting networks. A respective Lab-on-a-chip handling platform allows for the direct manipulation on a microscope stage and an incubator-free cell culture. Furthermore, luminescent sensors represent promising tools to be embedded into the microfluidic system to monitor the on-chip conditions or to provide information on cell viability and metabolic activity. Finally, examples for implemented assays on chip will be presented, ranging from cell culture showing the cell behavior in respect to surface functionalization and different growth conditions to finally embedding organ-on-chip structures of cultured cell lines.

Paper Details

Date Published: 13 May 2015
PDF: 6 pages
Proc. SPIE 9487, Smart Biomedical and Physiological Sensor Technology XII, 948704 (13 May 2015); doi: 10.1117/12.2178690
Show Author Affiliations
Claudia Gärtner, microfluidic ChipShop GmbH (Germany)
Birgit Ungerböck, microfluidic ChipShop GmbH (Germany)
Ingo Schulz, microfluidic ChipShop GmbH (Germany)
Tobias Jahn, microfluidic ChipShop GmbH (Germany)
Alexander Mosig, Jena Univ. Hospital (Germany)
Torsten Mayr, Graz Univ. of Technology (Austria)
Holger Becker, microfluidic ChipShop GmbH (Germany)

Published in SPIE Proceedings Vol. 9487:
Smart Biomedical and Physiological Sensor Technology XII
Brian M. Cullum; Eric S. McLamore, Editor(s)

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