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Localised hydrogen peroxide sensing for reproductive health
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Paper Abstract

The production of reactive oxygen species (ROS) is known to affect the developmental competence of embryos. Hydrogen peroxide (H2O2) an important reactive oxygen species, is also known to causes DNA damage and defective sperm function. Current techniques require incubating a developing embryo with an organic fluorophore which is potentially hazardous for the embryo. What we need is a localised ROS sensor which does not require fluorophores in solution and hence will allow continuous monitoring of H2O2 production without adversely affect the development of the embryo. Here we report studies on such a fibre-based sensor for the detection of H2O2 that uses a surface-bound aryl boronate fluorophore carboxyperoxyfluor-1(CPF1). Optical fibres present a unique platform due to desirable characteristics as dip sensors in biological solutions. Attempts to functionalise the fibre tips using polyelectrolyte layers and (3-aminopropyl)triethoxysilane (APTES) coatings resulted in a limited signal and poor fluorescent response to H2O2 due to a low tip surface density of the fluorophore. To increase the surface density, CPF1 was integrated into a polymer matrix formed on the fibre tip by a UV-catalysed polymerisation process of acrylamide onto a methacrylate silane layer. The polyacrylamide containing CPF1 gave a much higher surface density than previous surface attachment methods and the sensor was found to effectively detect H2O2. Using this method, biologically relevant concentrations of H2O2 were detected, enabling remote sensing studies into ROS releases from embryos throughout early development.

Paper Details

Date Published: 5 May 2015
PDF: 10 pages
Proc. SPIE 9506, Optical Sensors 2015, 950614 (5 May 2015); doi: 10.1117/12.2178680
Show Author Affiliations
Malcolm S. Purdey, The Univ. of Adelaide (Australia)
Erik P. Schartner, The Univ. of Adelaide (Australia)
Melanie L. Sutton-McDowall, The Univ. of Adelaide (Australia)
Lesley J. Ritter, The Univ. of Adelaide (Australia)
Jeremy Thompson, The Univ. of Adelaide (Australia)
ARC Ctr. of Excellence for Nanoscale BioPhotonics (Australia)
Tanya M. Monro, The Univ. of Adelaide (Australia)
Univ. of South Australia (Australia)
Andrew D. Abell, The Univ. of Adelaide (Australia)

Published in SPIE Proceedings Vol. 9506:
Optical Sensors 2015
Francesco Baldini; Jiri Homola; Robert A. Lieberman, Editor(s)

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