Fluorescence microscopy is widely used in various of practical applications now. High resolution optical sectional microscopic imaging utilized by confocal two- or multi-photon fluorescence microscopy has became an essential tool in biological researches. However, optical aberrations introduced by nonhomogeneity refractive index of tissues degraded the resolution and brightness of the images. Here we present the implementation of self-interference digital holographic adaptive optics in fluorescence microscopy. Wavefront sensing and correction is achieved by holographic recording and numerical processing approach, dispenses with Shack-Hartmann sensor and deformable mirror-based complicated system. The operation speed of the system is enhanced using off-axis Fourier triangular holography. Both the influence of the size and axial position of the guide star on the quality of the corrected images are investigated.

Date Published: 8 May 2015
PDF: 6 pages
Proc. SPIE 9508, Holography: Advances and Modern Trends IV, 950804 (8 May 2015); doi: 10.1117/12.2178639

Published in SPIE Proceedings Vol. 9508:
Holography: Advances and Modern Trends IV
Miroslav Hrabovský; John T. Sheridan; Antonio Fimia, Editor(s)