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Proceedings Paper

Impedance spectroscopy for detection of mold in archives with an integrated reference measurement
Author(s): P. Papireddy Vinayaka; S. Van Den Driesche; S. Janssen; M. Frodl; R. Blank; F. Cipriani; W. Lang; M.J. Vellekoop
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Paper Abstract

In this work, we present a new miniaturized culture medium based sensor system where we apply an optical reference in an impedance measurement approach for the detection of mold in archives. The designed sensor comprises a chamber with pre-loaded culture medium which promotes the growth of archive mold species. Growth of mold is detected by measuring changes in the impedance of the culture medium caused due to increase in the pH (from 5.5 to 8) with integrated electrodes. Integration of the reference measurement helps in determining the sensitivity of the sensor. The colorimetric principle serves as a reference measurement that indicates a pH change after which further pH shifts can be determined using impedance measurement. In this context, some of the major archive mold species Eurotium amstelodami, Aspergillus penicillioides and Aspergillus restrictus have been successfully analyzed on-chip. Growth of Eurotium amstelodami shows a proportional impedance change of 10 % (12 chips tested) per day, with a sensitivity of 0.6 kΩ/pH unit.

Paper Details

Date Published: 1 June 2015
PDF: 6 pages
Proc. SPIE 9518, Bio-MEMS and Medical Microdevices II, 95180T (1 June 2015); doi: 10.1117/12.2178510
Show Author Affiliations
P. Papireddy Vinayaka, Univ. of Bremen (Germany)
Microsystems Ctr. Bremen (Germany)
S. Van Den Driesche, Univ. of Bremen (Germany)
Microsystems Ctr. Bremen (Germany)
S. Janssen, Univ. of Bremen (Germany)
Microsystems Ctr. Bremen (Germany)
M. Frodl, microFAB Bremen GmbH (Germany)
R. Blank, Univ. of Bremen (Germany)
Microsystems Ctr. Bremen (Germany)
F. Cipriani, Univ. of Bremen (Germany)
W. Lang, Univ. of Bremen (Germany)
Microsystems Ctr. Bremen (Germany)
M.J. Vellekoop, Univ. of Bremen (Germany)
Microsystems Ctr. Bremen (Germany)

Published in SPIE Proceedings Vol. 9518:
Bio-MEMS and Medical Microdevices II
Sander van den Driesche, Editor(s)

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