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Proceedings Paper

Fluorescence imaging and spectroscopy of motile sperm cells and CHO cells in an optical trap (laser tweezers)
Author(s): Karsten Koenig; Yagang Liu; Tatiana B. Krasieva; Pasquale Patrizio; Yona Tadir M.D.; Gregory J. Sonek; Michael W. Berns; Bruce J. Tromberg
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Paper Abstract

We describe fluorescence spectroscopy and imaging studies of optically trapped single Chinese hamster ovary (CHO) and motile human sperm cells. The NIR trapping beam was provided by a tunable, multimode continuous wave Ti:Sapphire laser. The beam was introduced into an inverted confocal laser scanning microscope. Fluorescence of cells in the single- beam gradient force optical trap was excited with a 488 nm microbeam (laser scanning microscopy) or with 365 nm radiation from a high- pressure mercury lamp. Modifications to NADH-attributed autofluorescence and Rhodamine- and Propidium Iodide-attributed xenofluorescence indicate a significant cell-damaging effect of 760 nm trapping beams. 760 nm effects produce a biological response comparable to UVA-induced oxidative stress and appear to be a consequence to two-photon absorption.

Paper Details

Date Published: 22 May 1995
PDF: 12 pages
Proc. SPIE 2391, Laser-Tissue Interaction VI, (22 May 1995); doi: 10.1117/12.209888
Show Author Affiliations
Karsten Koenig, Institute for Molecular Biotechnology (Germany)
Beckman Laser Institute and Medical Clinic (United States)
Yagang Liu, Beckman Laser Institute and Medical Clinic and Univ. of California/Irvine (United States)
Tatiana B. Krasieva, Beckman Laser Institute and Medical Clinic (United States)
Pasquale Patrizio, Univ. of California/Irvine (United States)
Yona Tadir M.D., Institute for Molecular Biotechnology (Germany)
Gregory J. Sonek, Univ. of California/Irvine (United States)
Michael W. Berns, Beckman Laser Institute and Medical Clinic (United States)
Bruce J. Tromberg, Beckman Laser Institute and Medical Clinic (United States)

Published in SPIE Proceedings Vol. 2391:
Laser-Tissue Interaction VI
Steven L. Jacques, Editor(s)

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