Share Email Print
cover

Proceedings Paper

Radiometric calibration to consider in quantitative clinical fluorescence imaging measurements
Author(s): M. Litorja; A. Urbas; Y. Zong
Format Member Price Non-Member Price
PDF $17.00 $21.00

Paper Abstract

The fluorescent light detected by a clinical imager is assumed to be proportional only to the amount of fluorescent substance present in the sample and the level of excitation. Unfortunately, there are many factors that can add or subtract to the light signal directly attributable to the desired fluorescence emission, especially with fluorescence from inside the body imaged remotely. The quantification of fluorescence emission is feasible by calibrating the imager using international system of units (SI)-traceable physical and material calibration artifacts such that the detector’s digital numbers (DN) can be converted to radiometric units. Here we discuss three calibration methods for quantitative clinical fluorescence imaging systems.

Paper Details

Date Published: 4 March 2015
PDF: 4 pages
Proc. SPIE 9311, Molecular-Guided Surgery: Molecules, Devices, and Applications, 931114 (4 March 2015); doi: 10.1117/12.2086838
Show Author Affiliations
M. Litorja, National Institute of Standards and Technology (United States)
A. Urbas, National Institute of Standards and Technology (United States)
Y. Zong, National Institute of Standards and Technology (United States)


Published in SPIE Proceedings Vol. 9311:
Molecular-Guided Surgery: Molecules, Devices, and Applications
Brian W. Pogue; Sylvain Gioux, Editor(s)

© SPIE. Terms of Use
Back to Top
PREMIUM CONTENT
Sign in to read the full article
Create a free SPIE account to get access to
premium articles and original research
Forgot your username?
close_icon_gray