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Proceedings Paper

Holographic quantitative imaging of sample hidden by turbid medium or occluding objects
Author(s): V. Bianco; L. Miccio; F. Merola; P. Memmolo; O. Gennari; Melania Paturzo; P. A. Netti; P. Ferraro
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Paper Abstract

Digital Holography (DH) numerical procedures have been developed to allow imaging through turbid media. A fluid is considered turbid when dispersed particles provoke strong light scattering, thus destroying the image formation by any standard optical system. Here we show that sharp amplitude imaging and phase-contrast mapping of object hidden behind turbid medium and/or occluding objects are possible in harsh noise conditions and with a large field-of view by Multi-Look DH microscopy. In particular, it will be shown that both amplitude imaging and phase-contrast mapping of cells hidden behind a flow of Red Blood Cells can be obtained. This allows, in a noninvasive way, the quantitative evaluation of living processes in Lab on Chip platforms where conventional microscopy techniques fail. The combination of this technique with endoscopic imaging can pave the way for the holographic blood vessel inspection, e.g. to look for settled cholesterol plaques as well as blood clots for a rapid diagnostics of blood diseases.

Paper Details

Date Published: 11 March 2015
PDF: 5 pages
Proc. SPIE 9336, Quantitative Phase Imaging, 933612 (11 March 2015); doi: 10.1117/12.2086808
Show Author Affiliations
V. Bianco, Istituto di Cibernetica "E. Caianiello" (Italy)
L. Miccio, Istituto di Cibernetica "E. Caianiello" (Italy)
F. Merola, Istituto di Cibernetica "E. Caianiello" (Italy)
P. Memmolo, Istituto di Cibernetica "E. Caianiello" (Italy)
Istituto Italiano di Technologia (Italy)
O. Gennari, Istituto di Cibernetica "E. Caianiello" (Italy)
Melania Paturzo, Istituto di Cibernetica "E. Caianiello" (Italy)
P. A. Netti, Istituto Italiano di Tecnologia (Italy)
P. Ferraro, Istituto di Cibernetica "E. Caianiello" (Italy)

Published in SPIE Proceedings Vol. 9336:
Quantitative Phase Imaging
Gabriel Popescu; YongKeun Park, Editor(s)

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