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Proceedings Paper

Lifetime-based sensing in highly scattering media
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Paper Abstract

Fluorescence lifetime-based sensing is now recognized as a valuable methodology in clinical and analytical chemistry. For clinical chemistry, or for non-invasive sensing through skin, it is often necessary to obtain quantitative information in highly scattering media. Lifetime-based sensing can be used to obtain quantitative measurements in turbid media. We describe frequency-domain lifetime measurements in intralipid suspension, and present a reliable technique which allows phase-modulation lifetime measurements of fluorophores dispersed within or localized within intralipid. Lifetimes can be measured using an intensity decay law which accounts for the time delays and pulse-broadening effects of multiple light scattering events occurring in the intralipid. Alternatively, the phase and modulation measurements can be performed relative to a reference fluorophore of known lifetime. This approach provided reliable lifetime data for a pH-sensitive fluorophore contained within a microcuvette 4 mm under the surface of an intralipid suspension.

Paper Details

Date Published: 8 May 1995
PDF: 12 pages
Proc. SPIE 2388, Advances in Fluorescence Sensing Technology II, (8 May 1995); doi: 10.1117/12.208549
Show Author Affiliations
Henryk Szmacinski, Univ. of Maryland School of Medicine (United States)
Joseph R. Lakowicz, Univ. of Maryland School of Medicine (United States)

Published in SPIE Proceedings Vol. 2388:
Advances in Fluorescence Sensing Technology II
Joseph R. Lakowicz, Editor(s)

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