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Proceedings Paper

Fiber optic evanescent wave spectroscopy (FEWS) and its applications for multicomponent analysis of blood and biological fluids
Author(s): Ronit Simhi; David Bunimovich; Ben-Ami Sela; Abraham Katzir
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Paper Abstract

In numerous cases the technique of Fiberoptic Evanescent Wave Spectroscopy (FEWS) offers great advantages over regular IR spectroscopic methods. It provides an easy way for measuring the absorption spectra of highly absorbing or highly scattering samples. With FEWS one can perform measurements in situ and in real time and this is potentially useful for measurements on biological samples or for the monitoring of chemical reactions. In the present work, the FEWS technique was used to analyze human blood serum using a Fourier Transform Infrared Spectrometer. A special cell based on IR transmitting non-toxic silver halide fibers was designed. Further improvements in the analysis were obtained by adopting some multivariate calibration techniques that have already been used in clinical chemistry. The blood constituents analyzed were: urea, total protein, cholesterol, uric acid and calcium. Good agreement between our results and the ordinary chemical and enzymatic methods was obtained.

Paper Details

Date Published: 8 May 1995
PDF: 8 pages
Proc. SPIE 2388, Advances in Fluorescence Sensing Technology II, (8 May 1995); doi: 10.1117/12.208526
Show Author Affiliations
Ronit Simhi, Tel Aviv Univ. (Israel)
David Bunimovich, Tel Aviv Univ. (Israel)
Ben-Ami Sela, Sheba Medical Ctr. (Israel)
Abraham Katzir, Tel Aviv Univ. (Israel)

Published in SPIE Proceedings Vol. 2388:
Advances in Fluorescence Sensing Technology II
Joseph R. Lakowicz, Editor(s)

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