Here we wish to present automated laser spectrofluorimeter and some applications of it. The excitation part of the spectrofluorimeter includes an atmospheric pressure nitrogen laser (pulse duration 0.35 ns, peak power 350 kW) and distributed-feedback dye laser (tuning range 400 - 750 nm, spectral width 0.1 - 0.6 nm). It is possible to excite the probe by nitrogen laser or dye laser. The signal of fluorescence is registered by boxcarintegrator and data are input into the computer IBM PC/AT-286. Laser spectrofluorimeter is full automated, lasers, all optical and electronic systems are controlled by the computer. The laser spectrofluorimeter can measure time-resolved fluorescence characteristics with temporal resolution from subnanoseconds to milliseconds: (1) instantaneous fluorescence spectra (0.2 ns - 10 ms), (2) instantaneous anisotropy spectra and anisotropy kinetics (0.2 ns - 10 ms), (3) fluorescence kinetics and lifetime of excited state (0.05 ns - 10 ms). Experimental data can be treated with a full set of programs: (1) deconvolution of fluorescence pulse with up to 3 exponential approximation, (2) calculation of anisotropy kinetics with simultaneous deconvolution for different polarization, (3) calculation of instantaneous spectra from fluorescence decays, (4) mathematical treating of spectra (position of spectrum, halfwidth, asymmetry etc.).

Date Published: 8 May 1995
PDF: 9 pages
Proc. SPIE 2388, Advances in Fluorescence Sensing Technology II, (8 May 1995); doi: 10.1117/12.208521

Published in SPIE Proceedings Vol. 2388:
Advances in Fluorescence Sensing Technology II
Joseph R. Lakowicz, Editor(s)