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Proceedings Paper

Laser-induced fluorescence spectroscopy of colonic dysplasia: prospects for optical histological analysis
Author(s): Ramasamy Manoharan; George I. Zonios; Robert M. Cothren Jr.; Joseph Arendt; Jacques Van Dam; Michael S. Feld
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Paper Abstract

Several groups have shown that laser-induced fluorescence spectroscopy can detect dysplastic changes in human colon tissues. We present an approach based on analysis of the underlying tissue microstructure for extracting histological information from such spectral signals. The method employs fluorescence microscopy and tissue optics to model the `bulk' fluorescence collected with an optical fiber probe in a clinical setting. For both colonic normal and adenoma, we measured the intrinsic fluorescence lineshapes, the spatial distributions of the fluorophores, and optical parameters of tissue. Numerical and analytical solutions to the radiative transfer equation were then used to compute fluorescence spectra. The results of the model were in excellent agreement with clinical spectra collected during colonoscopy, using 370 nm excitation. Four factors were found to be responsible for the spectral differences between normal tissue and adenoma: fluorescence of mucosal collagen, dysplastic cell, and submucosa, and hemoglobin attenuation. Preliminary results indicate that these parameters can be extracted from individual clinical spectra by reversing the modeling procedure.

Paper Details

Date Published: 8 May 1995
PDF: 6 pages
Proc. SPIE 2388, Advances in Fluorescence Sensing Technology II, (8 May 1995); doi: 10.1117/12.208501
Show Author Affiliations
Ramasamy Manoharan, Massachusetts Institute of Technology (United States)
George I. Zonios, Massachusetts Institute of Technology (United States)
Robert M. Cothren Jr., Cleveland Clinic Foundation (United States)
Joseph Arendt, Cleveland Clinic Foundation (United States)
Jacques Van Dam, Brigham and Women's Hospital (United States)
Michael S. Feld, Massachusetts Institute of Technology (United States)

Published in SPIE Proceedings Vol. 2388:
Advances in Fluorescence Sensing Technology II
Joseph R. Lakowicz, Editor(s)

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