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Proceedings Paper

Immunoassays for pesticide monitoring
Author(s): Ingrid Wengatz; Ferenc Szurdoki; Anand R. Swamy; Lawrence Evans III; Gabor Patonay; Eric Stimmann; Michael Delwiche; Donald Stoutamire; Shirley J. Gee; Bruce D. Hammock
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Paper Abstract

This study compares two formats of rapid assays for the detection of pesticides (bromacil and pyrethroid based metabolites): enzyme linked immunosorbent assay (ELISA) and immunoassay with near-infrared (NIR) fluorescence detection. NIR dye immunoassay (NIRDIA) measurements were carried out by using two different instruments, both having a silicon photodiode as the detector and a laser diode for excitation. ELISA and NIRDIA were performed in a tracer format, where the specific antibody is bound to the surface of a microtiter plate well and the tracer with enzyme or fluorescent dye label competes with the analyte for the antibody binding site. It was demonstrated that the NIRDIA is at least as sensitive as the ELISA. Both assays detect pesticides in the (mu) g/L (ppb) range. Hapten- macromolecule-NIR dye-conjugates have been synthesized with various biopolymers (e.g., proteins) as carriers. The use of carrier macromolecules enables convenient purification of the cyanine dye derivatives. The mild conjugation method of the dye is based on isothiocyanate chemistry.

Paper Details

Date Published: 8 May 1995
PDF: 9 pages
Proc. SPIE 2388, Advances in Fluorescence Sensing Technology II, (8 May 1995); doi: 10.1117/12.208500
Show Author Affiliations
Ingrid Wengatz, Univ. of California/Davis (United States)
Ferenc Szurdoki, Univ. of California/Davis (United States)
Anand R. Swamy, Georgia State Univ. (United States)
Lawrence Evans III, Georgia State Univ. (United States)
Gabor Patonay, Georgia State Univ. (United States)
Eric Stimmann, Univ. of California/Davis (United States)
Michael Delwiche, Univ. of California/Davis (United States)
Donald Stoutamire, Univ. of California/Davis (United States)
Shirley J. Gee, Univ. of California/Davis (United States)
Bruce D. Hammock, Univ. of California/Davis (United States)

Published in SPIE Proceedings Vol. 2388:
Advances in Fluorescence Sensing Technology II
Joseph R. Lakowicz, Editor(s)

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