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Proceedings Paper

Sensitive laser wave-mixing detection methods for biomedical applications
Author(s): Jon A. Nunes; Sandrine Berniolles; William M. Tong
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Paper Abstract

Four-wave mixing is presented as an ultrasensitive detection method for liquid analytes in capillary flow cells and for circular dichroism measurements. A two-input-beam forward- scattering degenerate four-wave mixing optical setup offers simplified optical alignment, effective use of compact low-power lasers, and convenient interface to powerful chemical separation methods using capillary columns. The use of a single focusing lens for both input beams provides very high wave-mixing efficiency and tight focusing inside a small detection probe volume. Attomole or better detection sensitivity levels of this nonlinear laser detector compare favorably to those of many conventional laser-based analytical methods, yet this nonlinear detector can detect both fluorescing and non-fluorescing analytes. One can conveniently interface this multi-wave mixing detector to liquid chromatography and capillary electrophoresis for on-column detection. Nonlinear wave-mixing as a sensitive `direct' or `indirect' absorbance detection method offers important advantages in many biomedical applications.

Paper Details

Date Published: 8 May 1995
PDF: 8 pages
Proc. SPIE 2388, Advances in Fluorescence Sensing Technology II, (8 May 1995); doi: 10.1117/12.208479
Show Author Affiliations
Jon A. Nunes, San Diego State Univ. (United States)
Sandrine Berniolles, San Diego State Univ. (United States)
William M. Tong, San Diego State Univ. (United States)

Published in SPIE Proceedings Vol. 2388:
Advances in Fluorescence Sensing Technology II
Joseph R. Lakowicz, Editor(s)

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