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Proceedings Paper

New near-infrared dyes for applications in bioanalytical methods
Author(s): Narasimhachari Narayanan; Garrick Little; Ramesh Raghavachari; Gabor Patonay
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Paper Abstract

The near infrared (NIR) fluorescence has several advantages as a detection system over visible or uv fluorescence counterpart. The spectral properties of cyanine dyes offer advantages for detection in the longer wavelength NIR region. The present work focuses on the synthesis of NIR dyes and their applications in bioanalytical chemistry. Several cyanine dyes have been synthesized. The spectroscopic characteristics such as molar absorptivity, fluorescence quantum yield, excitation and emission maxima have been determined. The potential utilities of these new dyes as probe and reporter molecules in bioanalytical methods have been explored. Dyes bearing reactive functional groups such as isothiocyanate, carboxylic acid or hydroxyl groups can be directly used for covalent labeling of proteins and nucleic acids. Their importance in the synthesis of dye labeled phosphoramidites and dye labeled dideoxynucleotides and use in DNA sequencing have been evaluated.

Paper Details

Date Published: 8 May 1995
PDF: 10 pages
Proc. SPIE 2388, Advances in Fluorescence Sensing Technology II, (8 May 1995); doi: 10.1117/12.208471
Show Author Affiliations
Narasimhachari Narayanan, LI-COR, Inc. (United States)
Garrick Little, LI-COR, Inc. (United States)
Ramesh Raghavachari, LI-COR, Inc. (United States)
Gabor Patonay, Georgia State Univ. (United States)

Published in SPIE Proceedings Vol. 2388:
Advances in Fluorescence Sensing Technology II
Joseph R. Lakowicz, Editor(s)

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