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Proceedings Paper

Lifetime-based identification of single molecules
Author(s): Joerg Enderlein; Rolf Krahl; Uwe Ortmann; Michael Wahl; Rainer Erdmann; Edgar O. Klose
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Paper Abstract

Our new developed time-correlated single photon counting electronics allows for continuous registration of time-resolved fluorescence signals in millisecond intervals. With this electronics, adapted fluorescence measurement system, we studied single molecule transitions through a focused laser beam. In our paper we present measurements on single rhodamine 6G- molecules and discuss data processing methods, using the full information of the time resolved measurement. The use of this information influences significantly the reliability of the detection of single molecule transits. Further developments and applications of this method are discussed.

Paper Details

Date Published: 8 May 1995
PDF: 8 pages
Proc. SPIE 2388, Advances in Fluorescence Sensing Technology II, (8 May 1995); doi: 10.1117/12.208465
Show Author Affiliations
Joerg Enderlein, BiosQuant GmbH Berlin (Germany)
Rolf Krahl, BiosQuant GmbH Berlin (Germany)
Uwe Ortmann, BiosQuant GmbH Berlin (Germany)
Michael Wahl, BiosQuant GmbH Berlin (Germany)
Rainer Erdmann, BiosQuant GmbH Berlin (Germany)
Edgar O. Klose, BiosQuant GmbH Berlin (Germany)

Published in SPIE Proceedings Vol. 2388:
Advances in Fluorescence Sensing Technology II
Joseph R. Lakowicz, Editor(s)

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