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Proceedings Paper

Application of fluorescence spectroscopy and multispectral imaging for non-invasive estimation of GFP transfection efficiency
Author(s): M. Tamošiūnas; D. Jakovels; A. Ļihačovs; A. Kilikevičius; J. Baltušnikas; R. Kadikis; S. Šatkauskas
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Paper Abstract

Electroporation and ultrasound induced sonoporation has been showed to induce plasmid DNA transfection to the mice tibialis cranialis muscle. It offers new prospects for gene therapy and cancer treatment. However, numerous experimental data are still needed to deliver the plausible explanation of the mechanisms governing DNA electro- or sono-transfection, as well as to provide the updates on transfection protocols for transfection efficiency increase. In this study we aimed to apply non-invasive optical diagnostic methods for the real time evaluation of GFP transfection levels at the reduced costs for experimental apparatus and animal consumption. Our experimental set-up allowed monitoring of GFP levels in live mice tibialis cranialis muscle and provided the parameters for DNA transfection efficiency determination.

Paper Details

Date Published: 22 October 2014
PDF: 10 pages
Proc. SPIE 9421, Eighth International Conference on Advanced Optical Materials and Devices (AOMD-8), 94210M (22 October 2014); doi: 10.1117/12.2083895
Show Author Affiliations
M. Tamošiūnas, Vytautas Magnus Univ. (Lithuania)
D. Jakovels, Univ. of Latvia (Latvia)
A. Ļihačovs, Univ. of Latvia (Latvia)
A. Kilikevičius, Lithuanian Sports Univ. (Lithuania)
J. Baltušnikas, Lithuanian Sports Univ. (Lithuania)
R. Kadikis, Institute of Electronics and Computer Science (Latvia)
S. Šatkauskas, Vytautas Magnus Univ. (Lithuania)

Published in SPIE Proceedings Vol. 9421:
Eighth International Conference on Advanced Optical Materials and Devices (AOMD-8)
Janis Spigulis, Editor(s)

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