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Proceedings Paper

Impact of blue LED irradiation on proliferation and gene expression of cultured human keratinocytes
Author(s): Anja Becker; Carsten Sticht; Harsh Dweep; Frank A. van Abeelen; Norbert Gretz; Gerrit Oversluizen
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Paper Abstract

Blue light is known for its anti-microbial, anti-proliferative and anti-inflammatory effects. Furthermore, it is already used for the treatment of neonatal jaundice and acne. However, little is known about the exact mechanisms of action on gene expression level. The aim of this study was to assess the impact of blue LED irradiation on the proliferation and gene expression in immortalized human keratinocytes (HaCaT) in vitro. Furthermore its safety was assessed. XTT-tests revealed a decrease in cell proliferation in blue light irradiated cells depending on the duration of light irradiation. Moreover, gene expression analysis demonstrated deregulated genes already 3 hours after blue light irradiation. 24 hours after blue light irradiation the effects seemed to be even more pronounced. The oxidative stress response was significantly increased, pointing to increased ROS production due to blue light, as well as steroid hormone biosynthesis. Downregulated pathways or biological processes were connected to anti-inflammatory response. Interestingly, also the melanoma pathway contained significantly downregulated genes 24 hours after blue light irradiation, which stands in accordance to literature that blue light can also inhibit proliferation in cancer cells. First tests with melanoma cells revealed a decrease in cell proliferation after blue light irradiation. In conclusion, blue light irradiation might open avenues to new therapeutic regimens; at least blue light seems to have no effect that induces cancer growth or formation.

Paper Details

Date Published: 5 March 2015
PDF: 12 pages
Proc. SPIE 9309, Mechanisms for Low-Light Therapy X, 930909 (5 March 2015); doi: 10.1117/12.2083010
Show Author Affiliations
Anja Becker, Ruprecht-Karls-Univ. Heidelberg (Germany)
Carsten Sticht, Ruprecht-Karls-Univ. Heidelberg (Germany)
Harsh Dweep, Ruprecht-Karls-Univ. Heidelberg (Germany)
Frank A. van Abeelen, Philips Research (Netherlands)
Norbert Gretz, Ruprecht-Karls-Univ. Heidelberg (Germany)
Gerrit Oversluizen, Philips Research Nederland B.V. (Netherlands)

Published in SPIE Proceedings Vol. 9309:
Mechanisms for Low-Light Therapy X
Michael R. Hamblin; James D. Carroll; Praveen Arany, Editor(s)

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