
Proceedings Paper
Optical heterogeneous bioassay for the detection of the inflammatory biomarker suPARFormat | Member Price | Non-Member Price |
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Paper Abstract
Soluble urokinase plasminogen activator receptor (suPAR) is an inflammatory protein present in blood and a marker of
disease presence, severity and prognosis. A heterogeneous sandwich assay is proposed for quantifying suPAR by
employing a capture antibody from rat and a biotinylated detection antibody from mouse. Optical detection was achieved
by a successive exposure of the biotinylated sandwich to streptavidin labelled with ATTO647N. The heterogeneous
assay was implemented on a multichannel polymethylmetacrylate (PMMA) optical biochip, potentially capable of the
simultaneous detection of more than one analyte. Capture antibody was immobilized on the PMMA surface of the
microfluidic channel and the assay was performed with the following protocol: i) surface blocking with BSA, ii)
incubation with suPAR or PBS, iii) incubation with biotinylated suPAR detection Ab and iv) incubation with
streptavidin-ATTO647N. Promising preliminary results were obtained with this protocol. Moreover, an improved optical
setup is proposed which avoids the mechanical scanning of the chip and consequently the in-series fluorescence
excitation and read out, allowing the simultaneous measurement of the fluorescence on all the channels of the
microfluidic chip.
Paper Details
Date Published: 4 March 2015
PDF: 6 pages
Proc. SPIE 9313, Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XIII, 93130W (4 March 2015); doi: 10.1117/12.2082804
Published in SPIE Proceedings Vol. 9313:
Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XIII
Anita Mahadevan-Jansen; Tuan Vo-Dinh; Warren S. Grundfest M.D.; Quan Liu, Editor(s)
PDF: 6 pages
Proc. SPIE 9313, Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XIII, 93130W (4 March 2015); doi: 10.1117/12.2082804
Show Author Affiliations
S. Tombelli, Istituto di Fisica Applicata Nello Carrara (Italy)
C. Trono, Istituto di Fisica Applicata Nello Carrara (Italy)
B. Adinolfi, Istituto di Fisica Applicata Nello Carrara (Italy)
F. Chiavaioli, Istituto di Fisica Applicata Nello Carrara (Italy)
A. Giannetti, Istituto di Fisica Applicata Nello Carrara (Italy)
J. Eugen-Olsen, Copenhagen Univ. Hospital Hvidovre (Denmark)
Virogates A/S (Denmark)
C. Trono, Istituto di Fisica Applicata Nello Carrara (Italy)
B. Adinolfi, Istituto di Fisica Applicata Nello Carrara (Italy)
F. Chiavaioli, Istituto di Fisica Applicata Nello Carrara (Italy)
A. Giannetti, Istituto di Fisica Applicata Nello Carrara (Italy)
J. Eugen-Olsen, Copenhagen Univ. Hospital Hvidovre (Denmark)
Virogates A/S (Denmark)
R. Bernini, Istituto per il Rilevamento Elettromagnetico dell'Ambiente (Italy)
Immacolata A. Grimaldi, IREA-CNR, Institute for Electromagnetic Sensing of the Environment (Italy)
G. Persichetti, Istituto per il Rilevamento Elettromagnetico dell'Ambiente (Italy)
G. Testa, Istituto per il Rilevamento Elettromagnetico dell'Ambiente (Italy)
F. Baldini, Istituto di Fisica Applicata Nello Carrara (Italy)
Immacolata A. Grimaldi, IREA-CNR, Institute for Electromagnetic Sensing of the Environment (Italy)
G. Persichetti, Istituto per il Rilevamento Elettromagnetico dell'Ambiente (Italy)
G. Testa, Istituto per il Rilevamento Elettromagnetico dell'Ambiente (Italy)
F. Baldini, Istituto di Fisica Applicata Nello Carrara (Italy)
Published in SPIE Proceedings Vol. 9313:
Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XIII
Anita Mahadevan-Jansen; Tuan Vo-Dinh; Warren S. Grundfest M.D.; Quan Liu, Editor(s)
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