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Proceedings Paper

Detection of small biological objects by phase-sensitive optical coherence tomography
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Paper Abstract

We demonstrate a novel label-free OCT method allowing optical detection and differentiation of moving micro-objects, such as blood cells. In this study we use phase-sensitive Fd-OCT/OCM system with broadband light source (axial resolution: 3 μm in tissue and lateral resolution 4−8 μm) and fluidic sample setup. The novel part of this method is that the optical identification is based on optical signal coming from optically uniform scattering media localized beneath the flowing/moving objects and not from the objects itself. This signal reveals as an enhancement in speckle pattern on intensity images and non-zero phase change on phase images (phase-change). Statistical parameters of such signals depend on the features of the object, like its size, shape, internal structure, etc. In order to demonstrate the effectiveness and reliability of proposed method, we performed an experiment to differentiate erythrocytes from leukocytes. Obtained OCT cross-sectional images present signal enhancement in the scattering base, both on intensity and phase-change images. This modulation signals, corresponding to erythrocytes and leukocytes, are significantly different and could be easily distinguished qualitatively. Statistical parameters used for the analysis also represent satisfactory separation to distinguish between different kinds of cells. Above-mentioned results of differentiation are presented in this paper.

Paper Details

Date Published: 2 March 2015
PDF: 5 pages
Proc. SPIE 9312, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XIX, 93122A (2 March 2015); doi: 10.1117/12.2082140
Show Author Affiliations
Paweł Ossowski, Nicolaus Copernicus Univ. (Poland)
AM2M LLC-LP (Poland)
Anna Raiter, AM2M LLC-LP (Poland)
Anna Szkulmowska, AM2M LLC-LP (Poland)
Maciej Wojtkowski, Nicolaus Copernicus Univ. (Poland)
AM2M LLC-LP (Poland)

Published in SPIE Proceedings Vol. 9312:
Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XIX
James G. Fujimoto; Joseph A. Izatt; Valery V. Tuchin, Editor(s)

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