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Proceedings Paper

Automated classification of bone marrow cells in microscopic images for diagnosis of leukemia: a comparison of two classification schemes with respect to the segmentation quality
Author(s): Sebastian Krappe; Michaela Benz; Thomas Wittenberg; Torsten Haferlach; Christian Münzenmayer
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Paper Abstract

The morphological analysis of bone marrow smears is fundamental for the diagnosis of leukemia. Currently, the counting and classification of the different types of bone marrow cells is done manually with the use of bright field microscope. This is a time consuming, partly subjective and tedious process. Furthermore, repeated examinations of a slide yield intra- and inter-observer variances. For this reason an automation of morphological bone marrow analysis is pursued. This analysis comprises several steps: image acquisition and smear detection, cell localization and segmentation, feature extraction and cell classification. The automated classification of bone marrow cells is depending on the automated cell segmentation and the choice of adequate features extracted from different parts of the cell. In this work we focus on the evaluation of support vector machines (SVMs) and random forests (RFs) for the differentiation of bone marrow cells in 16 different classes, including immature and abnormal cell classes. Data sets of different segmentation quality are used to test the two approaches. Automated solutions for the morphological analysis for bone marrow smears could use such a classifier to pre-classify bone marrow cells and thereby shortening the examination duration.

Paper Details

Date Published: 20 March 2015
PDF: 6 pages
Proc. SPIE 9414, Medical Imaging 2015: Computer-Aided Diagnosis, 94143I (20 March 2015); doi: 10.1117/12.2081946
Show Author Affiliations
Sebastian Krappe, Fraunhofer-Institut für Integrierte Schaltungen (IIS) (Germany)
Michaela Benz, Fraunhofer-Institut für Integrierte Schaltungen (IIS) (Germany)
Thomas Wittenberg, Fraunhofer-Institut für Integrierte Schaltungen (IIS) (Germany)
Torsten Haferlach, MLL Munich Leukemia Lab. (Germany)
Christian Münzenmayer, Fraunhofer-Institut für Integrierte Schaltungen (IIS) (Germany)

Published in SPIE Proceedings Vol. 9414:
Medical Imaging 2015: Computer-Aided Diagnosis
Lubomir M. Hadjiiski; Georgia D. Tourassi, Editor(s)

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