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Proceedings Paper

Effect of photodynamic therapy using benzoporphyrin derivative on the cutaneous immune response
Author(s): Guillermo O. Simkin; Modestus Obochi; David W. C. Hunt; Agnes H. Chan; Julia G. Levy
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Paper Abstract

In this study, the effect of transdermal photodynamic therapy (PDT) using benzoporphyrin derivative monoacid ring A (BPD) on the development of the immunologically mediated contact hypersensitivity (CHS) response against the hapten dinitrofluorobenzene (DNFB) and on the duration of skin allograft acceptance has been evaluated. In the CHS model it was found that the treatment of hairless strain mice with whole-body transdermal PDT using BPD (1 mg/kg) and LED light (15 J/cm2) resulted in a profound suppression of the CHS reaction if treatment was applied either 48 or 24 hours prior or up to 72 hours after sensitization of abdominal skin with DNFB. Less inhibition of the CHS response was observed if PDT was given one day before the ear challenge with DNFB which was applied 5 days following the initial DNFB sensitization. However, DNFB-exposed, PDT-treated mice retained the capacity to respond maximally to the unrelated contact sensitizer oxazolone. These results are consistent with other models of experimentally induced immune tolerance. allogeneic skin graft studies demonstrated that pretreatment of skin with BPD and light, at levels that did not cause significant tissue damage, significantly enhanced the length of engraftment. Using a separate protocol, photodynamic treatment of recipient mice at various times after transplant had no significant effect on allograft acceptance. Irradiation of skin in the presence of BPD may significantly inhibit the initiation of certain immunological responses within these tissues.

Paper Details

Date Published: 1 May 1995
PDF: 11 pages
Proc. SPIE 2392, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy IV, (1 May 1995); doi: 10.1117/12.208190
Show Author Affiliations
Guillermo O. Simkin, Quadra Logic Technologies, Inc. (Canada)
Modestus Obochi, Univ. of British Columbia (Canada)
David W. C. Hunt, Quadra Logic Technologies, Inc. (Canada)
Agnes H. Chan, Quadra Logic Technologies, Inc. (Canada)
Julia G. Levy, Univ. of British Columbia (Canada)

Published in SPIE Proceedings Vol. 2392:
Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy IV
Thomas J. Dougherty, Editor(s)

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