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Proceedings Paper

CINCH (confocal incoherent correlation holography) super resolution fluorescence microscopy based upon FINCH (Fresnel incoherent correlation holography)
Author(s): Nisan Siegel; Brian Storrie; Marc Bruce; Gary Brooker
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Paper Abstract

FINCH holographic fluorescence microscopy creates high resolution super-resolved images with enhanced depth of focus. The simple addition of a real-time Nipkow disk confocal image scanner in a conjugate plane of this incoherent holographic system is shown to reduce the depth of focus, and the combination of both techniques provides a simple way to enhance the axial resolution of FINCH in a combined method called "CINCH". An important feature of the combined system allows for the simultaneous real-time image capture of widefield and holographic images or confocal and confocal holographic images for ready comparison of each method on the exact same field of view. Additional GPU based complex deconvolution processing of the images further enhances resolution.

Paper Details

Date Published: 11 March 2015
PDF: 12 pages
Proc. SPIE 9336, Quantitative Phase Imaging, 93360S (11 March 2015); doi: 10.1117/12.2081319
Show Author Affiliations
Nisan Siegel, Johns Hopkins Univ. (United States)
Brian Storrie, Univ. of Arkansas for Medical Sciences (United States)
Marc Bruce, Microvolution (United States)
Gary Brooker, Johns Hopkins Univ. (United States)

Published in SPIE Proceedings Vol. 9336:
Quantitative Phase Imaging
Gabriel Popescu; YongKeun Park, Editor(s)

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