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Proceedings Paper

Experimental systems for optogenetic control of protein activity with photodissociable fluorescent proteins
Author(s): Xin X. Zhou; Michael Z. Lin
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Paper Abstract

Fluorescent Light-inducible Proteins are proteins of interest that have Dronpa domains fused to both termini. In the dark, the Dronpa domains tetramerize and cage the protein, but light induces Dronpa dissociation and activates the protein. Here, we describe four experimental systems for light activation of mammalian cells expressing FLiPs.

Paper Details

Date Published: 10 March 2015
PDF: 4 pages
Proc. SPIE 9305, Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics II, 930534 (10 March 2015); doi: 10.1117/12.2081242
Show Author Affiliations
Xin X. Zhou, Stanford Univ. (United States)
Michael Z. Lin, Stanford Univ. (United States)

Published in SPIE Proceedings Vol. 9305:
Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics II
Henry Hirschberg M.D.; E. Duco Jansen; Samarendra K. Mohanty; Nitish V. Thakor; Qingming Luo; Steen J. Madsen, Editor(s)

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