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Proceedings Paper

High-resolution polarization sensitive OCT for ocular imaging in rodents
Author(s): Stanislava Fialová; Sabine Rauscher; Marion Gröger; Michael Pircher; Christoph K. Hitzenberger; Bernhard Baumann
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Paper Abstract

A new high-resolution polarization sensitive optical coherence tomography system was developed for imaging rodent retina. Various light-tissue interactions such as birefringence and depolarization can change the polarization state of light. In the eye, there are several tissues that have these properties, for example retinal pigment epithelium (depolarization) and sclera (birefringence). These layers play key roles in diseases like age-related macular degeneration or glaucoma. Animal models are an important component for understanding disease pathogenesis. The gold standard for the evaluation of preclinical experiments is histology, which is an invasive and terminal procedure. Since OCT is non-invasive, it has the potential to be an alternative to histology with the benefit of long-term study of the disease progression in the same animal. In this study, a superluminescent diode with spectrum width 100 nm and mean wavelength 840 nm is used as a light source in order to enable high axial resolution. Spectrometers are custom built to enable high imaging speed that allows acquiring 3D data sets with 1024x200x1536 voxels in 3.44 s. From the acquired data, images displaying phase retardation induced by birefringence and orientation of birefringent axis were calculated. In first measurements, we were able to identify the RPE-choroid complex (depolarization effect) and the sclera (strong birefringence) in the retina of Long-Evans and Sprague-Dawley rats. Our preliminary results demonstrate the feasibility of the system for high speed/resolution imaging of the rodent retina. This is useful for longitudinal studies of disease models of retinal disease in rats and mice

Paper Details

Date Published: 2 March 2015
PDF: 4 pages
Proc. SPIE 9312, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XIX, 93120T (2 March 2015); doi: 10.1117/12.2081229
Show Author Affiliations
Stanislava Fialová, Medizinische Univ. Wien (Austria)
Sabine Rauscher, Medizinische Univ. Wien (Austria)
Marion Gröger, Medizinische Univ. Wien (Austria)
Michael Pircher, Medizinische Univ. Wien (Austria)
Christoph K. Hitzenberger, Medizinische Univ. Wien (Austria)
Bernhard Baumann, Medizinische Univ. Wien (Austria)

Published in SPIE Proceedings Vol. 9312:
Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XIX
James G. Fujimoto; Joseph A. Izatt; Valery V. Tuchin, Editor(s)

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