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Proceedings Paper

Simultaneous quadruple modal nonlinear optical imaging for gastric diseases diagnosis and characterization
Author(s): Zi Wang; Wei Zheng; Jian Lin; Zhiwei Huang
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Paper Abstract

We report the development of a unique simultaneous quadruple-modal nonlinear optical microscopy (i.e., stimulated Raman scattering (SRS), second-harmonic generation (SHG), two-photon excitation fluorescence (TPEF), and third-harmonic generation (THG)) platform for characterization of the gastric diseases (i.e., gastritis, intestinal metaplasia (IM), intestinal type adenocarcinoma). SRS highlights the goblet cells found in IM. SHG images the distribution of collagen in lamina propria. Collagen is found to aggregate for intestinal type adenocarcinoma. TPEF reveals the cell morphology and can reflect the damage inside glands caused by the diseases. THG visualizes the nuclei with high spatial resolution, which facilitates the identification of neutrophils that are usually used as a feature of inflammation. This work shows that the co-registration of quadruple-modal images can be an effective means for diagnosis and characterization of gastric diseases at the cellular and molecular levels.

Paper Details

Date Published: 5 March 2015
PDF: 6 pages
Proc. SPIE 9329, Multiphoton Microscopy in the Biomedical Sciences XV, 93291P (5 March 2015); doi: 10.1117/12.2080901
Show Author Affiliations
Zi Wang, National Univ. of Singapore (Singapore)
Wei Zheng, National Univ. of Singapore (Singapore)
Jian Lin, National Univ. of Singapore (Singapore)
Zhiwei Huang, National Univ. of Singapore (Singapore)

Published in SPIE Proceedings Vol. 9329:
Multiphoton Microscopy in the Biomedical Sciences XV
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

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