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Proceedings Paper

Optical cell cleaning with NIR femtosecond laser pulses
Author(s): Aisada Uchugonova; Hans Georg Breunig; Ana Batista; Karsten König
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Paper Abstract

Femtosecond laser microscopes have been used as both micro and nanosurgery tools. The optical knock-out of undesired cells in multiplex cell clusters shall be further reported on in this study.

Femtosecond laser-induced cell death is beneficial due to the reduced collateral side effects and therefore can be used to selectively destroy target cells within monolayers, as well as within 3D tissues, all the while preserving cells of interest. This is an important characteristic for the application in stem cell research and cancer treatment. Non-precise damage compromises the viability of neighboring cells by inducing side effects such as stress to the cells surrounding the target due to the changes in the microenvironment, resulting from both the laser and laser-exposed cells.

In this study, optimum laser parameters for optical cleaning by isolating single cells and cell colonies are exploited through the use of automated software control. Physiological equilibrium and cellular responses to the laser induced damages are also investigated. Cell death dependence on laser focus, determination and selectivity of intensity/dosage, controllable damage and cell recovery mechanisms are discussed.

Paper Details

Date Published: 2 March 2015
PDF: 5 pages
Proc. SPIE 9328, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIII, 932819 (2 March 2015); doi: 10.1117/12.2080867
Show Author Affiliations
Aisada Uchugonova, Saarland Univ. (Germany)
JenLab GmbH (Germany)
Hans Georg Breunig, Saarland Univ. (Germany)
JenLab GmbH (Germany)
Ana Batista, Saarland Univ. (Germany)
Karsten König, Saarland Univ. (Germany)
JenLab GmbH (Germany)

Published in SPIE Proceedings Vol. 9328:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIII
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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