
Proceedings Paper
Microscopy with UV Surface Excitation (MUSE) for slide-free histology and pathology imagingFormat | Member Price | Non-Member Price |
---|---|---|
$17.00 | $21.00 |
Paper Abstract
A novel microscopy method that takes advantage of shallow photon penetration using ultraviolet-range excitation and
exogenous fluorescent stains is described. This approach exploits the intrinsic optical sectioning function when exciting
tissue fluorescence from superficial layers to generate images similar to those obtainable from a physically thinsectioned
tissue specimen. UV light in the spectral range from roughly 240-275 nm penetrates only a few microns into
the surface of biological specimens, thus eliminating out-of-focus signals that would otherwise arise from deeper tissue
layers. Furthermore, UV excitation can be used to simultaneously excite fluorophores emitting across a wide spectral
range. The sectioning property of the UV light (as opposed to more conventional illumination in the visible range)
removes the need for physical or more elaborate optical sectioning approaches, such as confocal, nonlinear or coherent
tomographic methods, to generate acceptable axial resolution. Using a tunable laser, we investigated the effect of
excitation wavelength in the 230-350 nm spectral range on excitation depth. The results reveal an optimal wavelength
range and suggest that this method can be a fast and reliable approach for rapid imaging of tissue specimens. Some of
this range is addressable by currently available and relatively inexpensive LED light sources. MUSE may prove to be a
good alternative to conventional, time-consuming, histopathology procedures.
Paper Details
Date Published: 11 March 2015
PDF: 6 pages
Proc. SPIE 9318, Optical Biopsy XIII: Toward Real-Time Spectroscopic Imaging and Diagnosis, 93180F (11 March 2015); doi: 10.1117/12.2080408
Published in SPIE Proceedings Vol. 9318:
Optical Biopsy XIII: Toward Real-Time Spectroscopic Imaging and Diagnosis
Robert R. Alfano; Stavros G. Demos, Editor(s)
PDF: 6 pages
Proc. SPIE 9318, Optical Biopsy XIII: Toward Real-Time Spectroscopic Imaging and Diagnosis, 93180F (11 March 2015); doi: 10.1117/12.2080408
Show Author Affiliations
Farzad Fereidouni, Univ. of California, Davis (United States)
Ananya Datta Mitra, Univ. of California, Davis (United States)
Ananya Datta Mitra, Univ. of California, Davis (United States)
Stavros Demos, Lawrence Livermore National Lab. (United States)
Richard Levenson, Univ. of California, Davis (United States)
Richard Levenson, Univ. of California, Davis (United States)
Published in SPIE Proceedings Vol. 9318:
Optical Biopsy XIII: Toward Real-Time Spectroscopic Imaging and Diagnosis
Robert R. Alfano; Stavros G. Demos, Editor(s)
© SPIE. Terms of Use
