In this work, we experimentally determine the transfer function of our recently reported epi-illumination white light diffraction phase microscopy (epi-wDPM) system. The transfer function identifies how the low frequencies below k₀NA_con are modified due to the limited spatial coherence and how the high frequencies above k₀NA_obj are affected due to the limited objective numerical aperture. Using this transfer function, we perform deconvolution to remove the halo and obtain proper quantitative phase measurements without the need for excessive spatial filtering. The wDPM and epi-wDPM systems are now capable of obtaining halo-free images with proper topography at much higher speeds.

Date Published: 11 March 2015
PDF: 5 pages
Proc. SPIE 9336, Quantitative Phase Imaging, 933610 (11 March 2015); doi: 10.1117/12.2080318

Published in SPIE Proceedings Vol. 9336:
Quantitative Phase Imaging
Gabriel Popescu; YongKeun Park, Editor(s)