Brillouin and Raman microspectroscopies are powerful techniques for noninvasive optical imaging. In particular, Brillouin microscopy is a unique technique, which allows assessing sample’s mechanical properties with optical level spatial resolution. Recent advances in background-free Brillouin microspectroscopy make it possible to image turbid samples without substantial degradation of the data quality. In this report, we applied Brillouin spectroscopy to imaging individual cells. These viscoelastic measurements were supplemented by chemically specific measurements using confocal Raman microspectroscopy.

Date Published: 13 April 2015
PDF: 6 pages
Proc. SPIE 9328, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIII, 93280P (13 April 2015); doi: 10.1117/12.2080317

Published in SPIE Proceedings Vol. 9328:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIII
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)