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Proceedings Paper

Imaging highly absorbing nanoparticles using photothermal microscopy
Author(s): Simon-Alexandre Lussier; Hamid Moradi; Alain Price; Sangeeta Murugkar
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Paper Abstract

Gold nanoparticles (NPs) have tremendous potential in biomedicine. They can be used as absorbing labels inside living cells for the purpose of biomedical imaging, biosensing as well as for photothermal therapy. We demonstrate photothermal imaging of highly-absorbing particles using a pump-probe setup. The photothermal signal is recovered by heterodyne detection, where the excitation pump laser is at 532 nm and the probe laser is at 638 nm. The sample is moved by a scanning stage. Proof of concept images of red polystyrene microspheres and gold nanoparticles are obtained with this home-built multimodal microscope. The increase in temperature at the surface of the gold NPs, due to the pump laser beam, can be directly measured by means of this photothermal microscope and then compared with the results from theoretical predictions. This technique will be useful for characterization of nanoparticles of different shapes, sizes and materials that are used in cancer diagnosis and therapy.

Paper Details

Date Published: 11 March 2015
PDF: 5 pages
Proc. SPIE 9337, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XII, 93370N (11 March 2015); doi: 10.1117/12.2080278
Show Author Affiliations
Simon-Alexandre Lussier, Carleton Univ. (Canada)
Hamid Moradi, Carleton Univ. (Canada)
Alain Price, Carleton Univ. (Canada)
Sangeeta Murugkar, Carleton Univ. (Canada)

Published in SPIE Proceedings Vol. 9337:
Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XII
Alexander N. Cartwright; Dan V. Nicolau, Editor(s)

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