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Proceedings Paper

Molecule-specific darkfield and multiphoton imaging using gold nanocages
Author(s): Amy J. Powless; Samir V Jenkins; Mary Lee McKay; Jingyi Chen; Timothy J. Muldoon
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Paper Abstract

Due to their robust optical properties, biological inertness, and readily adjustable surface chemistry, gold nanostructures have been demonstrated as contrast agents in a variety of biomedical imaging applications. One application is dynamic imaging of live cells using bioconjugated gold nanoparticles to monitor molecule trafficking mechanisms within cells; for instance, the regulatory pathway of epidermal growth factor receptor (EGFR) undergoing endocytosis. In this paper, we have demonstrated a method to track endocytosis of EGFR in MDA-MB-468 breast adenocarcinoma cells using bioconjugated gold nanocages (AuNCs) and multiphoton microscopy. Dynamic imaging was performed using a time series capture of 4 images every minute for one hour. Specific binding and internalization of the bioconjugated AuNCs was observed while the two control groups showed non-specific binding at fewer surface sites, leading to fewer bound AuNCs and no internalization.

Paper Details

Date Published: 12 March 2015
PDF: 6 pages
Proc. SPIE 9339, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications VII, 93390B (12 March 2015); doi: 10.1117/12.2080152
Show Author Affiliations
Amy J. Powless, Univ. of Arkansas (United States)
Samir V Jenkins, Univ. of Arkansas (United States)
Mary Lee McKay, Univ. of Arkansas (United States)
Jingyi Chen, Univ. of Arkansas (United States)
Timothy J. Muldoon, Univ. of Arkansas (United States)

Published in SPIE Proceedings Vol. 9339:
Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications VII
Samuel Achilefu; Ramesh Raghavachari, Editor(s)

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