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Proceedings Paper

Multimodal optical phenotyping of cancer cells
Author(s): Lena Kastl; Björn Budde; Michael Isbach; Christina Rommel; Björn Kemper; Jürgen Schnekenburger
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Paper Abstract

There is a growing interest in label-free, optical techniques like digital holographic microscopy (DHM) and optical cell stretching, since the interaction with samples is minimized. Because optical manipulation strongly depends on the optical and physiological properties of the investigated material, we combined the usage of these methods for the characterization of pancreatic tumor cells. Our results demonstrate that cells of distinct differentiation levels, or different expression in only one protein, show differences in their deformability. Additionally, the DHM results showed only few variations in the refractive index, indicating that it does not significantly influence the results of the optical cell stretching. Thus, the combined usage of the two technologies represents a promising new approach for tumor cell characterization.

Paper Details

Date Published: 2 March 2015
PDF: 5 pages
Proc. SPIE 9328, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIII, 93280F (2 March 2015); doi: 10.1117/12.2080151
Show Author Affiliations
Lena Kastl, Univ. Münster (Germany)
Björn Budde, Univ. Münster (Germany)
Michael Isbach, Univ. Münster (Germany)
Christina Rommel, Univ. Münster (Germany)
Björn Kemper, Univ. Münster (Germany)
Jürgen Schnekenburger, Univ. Münster (Germany)

Published in SPIE Proceedings Vol. 9328:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIII
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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