Adaptive optics (AO) can potentially allow high resolution imaging deep inside living tissue, mitigating against the loss of resolution due to aberrations caused by overlying tissue. Closed-loop AO correction is particularly attractive for moving tissue and spatially varying aberrations, but this requires direct wavefront sensing, which in turn requires suitable "guide stars" for use as wavefront references. We present a novel method for generating an orthogonally illuminated guide star suitable for direct wavefront sensing in a wide range of fluorescent biological structures, along with results demonstrating its use for measuring time-varying aberrations, in vivo.

Date Published: 31 March 2015
PDF: 6 pages
Proc. SPIE 9335, Adaptive Optics and Wavefront Control for Biological Systems, 93350A (31 March 2015); doi: 10.1117/12.2080061

Published in SPIE Proceedings Vol. 9335:
Adaptive Optics and Wavefront Control for Biological Systems
Thomas G. Bifano; Joel Kubby; Sylvain Gigan, Editor(s)