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Proceedings Paper

Live dynamic imaging and analysis of developmental cardiac defects in mouse models with optical coherence tomography
Author(s): Andrew L. Lopez III; Shang Wang; Monica Garcia; Christian Valladolid; Kirill V. Larin; Irina V. Larina
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Paper Abstract

Understanding mouse embryonic development is an invaluable resource for our interpretation of normal human embryology and congenital defects. Our research focuses on developing methods for live imaging and dynamic characterization of early embryonic development in mouse models of human diseases. Using multidisciplinary methods: optical coherence tomography (OCT), live mouse embryo manipulations and static embryo culture, molecular biology, advanced image processing and computational modeling we aim to understand developmental processes. We have developed an OCT based approach to image live early mouse embryos (E8.5 – E9.5) cultured on an imaging stage and visualize developmental events with a spatial resolution of a few micrometers (less than the size of an individual cell) and a frame rate of up to hundreds of frames per second and reconstruct cardiodynamics in 4D (3D+time). We are now using these methods to study how specific embryonic lethal mutations affect cardiac morphology and function during early development.

Paper Details

Date Published: 10 March 2015
PDF: 5 pages
Proc. SPIE 9334, Optical Methods in Developmental Biology III, 93340S (10 March 2015); doi: 10.1117/12.2079951
Show Author Affiliations
Andrew L. Lopez III, Baylor College of Medicine (United States)
Shang Wang, Baylor College of Medicine (United States)
Monica Garcia, Baylor College of Medicine (United States)
Christian Valladolid, Baylor College of Medicine (United States)
Kirill V. Larin, Baylor College of Medicine (United States)
Univ. of Houston (United States)
Irina V. Larina, Baylor College of Medicine (United States)

Published in SPIE Proceedings Vol. 9334:
Optical Methods in Developmental Biology III
Andrew M. Rollins; Scott E. Fraser; Michael A. Choma M.D., Editor(s)

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