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Proceedings Paper

Noninvasive and label-free detection of circulating melanoma cells by in vivo photoacoustic flow cytometry
Author(s): Ping Yang; Rongrong Liu; Zhenyu Niu; Yuanzhen Suo; Hao He; Xunbin Wei
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Paper Abstract

Melanoma is a malignant tumor of melanocytes. Circulating melanoma cell has high light absorption due to melanin highly contained in melanoma cells. This property is employed for the detection of circulating melanoma cell by in vivo photoacoustic flow cytometry (PAFC). PAFC is based on photoacoustic effect. Compared to in vivo flow cytometry based on fluorescence, PAFC can employ high melanin content of melanoma cells as endogenous biomarkers to detect circulating melanoma cells in vivo. In our research, we developed in vitro experiments to prove the ability of PAFC system of detecting PA signals from melanoma cells. For in vivo experiments, we constructed a model of melanoma tumor bearing mice by inoculating highly metastatic murine melanoma cancer cells B16F10 with subcutaneous injection. PA signals were detected in the blood vessels of mouse ears in vivo. By counting circulating melanoma cells termly, we obtained the number variation of circulating melanoma cells as melanoma metastasized. Those results show that PAFC is a noninvasive and label-free method to detect melanoma metastases in blood or lymph circulation. Our PAFC system is an efficient tool to monitor melanoma metastases, cancer recurrence and therapeutic efficacy.

Paper Details

Date Published: 9 March 2015
PDF: 5 pages
Proc. SPIE 9324, Biophotonics and Immune Responses X, 93240G (9 March 2015); doi: 10.1117/12.2079907
Show Author Affiliations
Ping Yang, Shanghai Jiao Tong Univ. (China)
Rongrong Liu, Shanghai Jiao Tong Univ. (China)
Zhenyu Niu, Shanghai Jiao Tong Univ. (China)
Yuanzhen Suo, Shanghai Jiao Tong Univ. (China)
Hao He, Shanghai Jiao Tong Univ. (China)
Xunbin Wei, Shanghai Jiao Tong Univ. (China)

Published in SPIE Proceedings Vol. 9324:
Biophotonics and Immune Responses X
Wei R. Chen, Editor(s)

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