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Proceedings Paper

Origins of intracellular calcium mobilization evoked by infrared laser stimulation
Author(s): Cory A. Olsovsky; Gleb P. Tolstykh; Bennett L. Ibey; Hope T. Beier
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Paper Abstract

Cellular delivery of pulsed IR laser energy has been shown to stimulate action potentials in neurons. The mechanism for this stimulation is not completely understood. Certain hypotheses suggest the rise in temperature from IR exposure could activate temperature- or pressure-sensitive channels, or create pores in the cellular outer membrane. Studies using intensity-based Ca2+-responsive dyes show changes in Ca2+ levels after various IR stimulation parameters; however, determination of the origin of this signal proved difficult. An influx of larger, typically plasma-membrane-impermeant ions has been demonstrated, which suggests that Ca2+ may originate from the external solution. However, activation of intracellular signaling pathways, possibly indicating a more complex role of increasing Ca2+ concentration, has also been shown. By usingCa2+ sensitive dye Fura-2 and a high-speed ratiometric imaging system that rapidly alternates the excitation wavelengths, we have quantified the Ca2+ mobilization in terms of influx from the external solution and efflux from intracellular organelles. CHO-K1 cells, which lack voltage-gated Ca2+ channels, and NG-108 neuroblastoma cells, which do not produce action potentials in an early undifferentiated state, are used to determine the origin of the Ca2+ signals and investigate the role these mechanisms may play in IR neural stimulation.

Paper Details

Date Published: 5 March 2015
PDF: 5 pages
Proc. SPIE 9321, Optical Interactions with Tissue and Cells XXVI, 93210L (5 March 2015); doi: 10.1117/12.2079895
Show Author Affiliations
Cory A. Olsovsky, Texas A&M Univ. (United States)
Gleb P. Tolstykh, General Dynamics Information Technology (United States)
Bennett L. Ibey, Air Force Research Lab. (United States)
Hope T. Beier, Air Force Research Lab. (United States)

Published in SPIE Proceedings Vol. 9321:
Optical Interactions with Tissue and Cells XXVI
E. Duco Jansen, Editor(s)

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