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Proceedings Paper

Using SPIM to track the development of the focal power of the zebrafish lens
Author(s): Laura K. Young; Miguel Jarrin; Christopher D. Saunter; Roy Quinlan; John M. Girkin
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Paper Abstract

Selective plane illumination microscopy (SPIM) is a 3D imaging technique that uses a sheet of light to optically section a sample in vivo. A cylindrical lens focuses collimated light in one dimension, producing a sheet that is formed in the sample via an objective lens. Any optical power within the sample will additionally refract the light sheet passing through it. We exploit this effect to track the development of the optical power of the zebrafish lens over the first 4 days post fertilisation (dpf). We show that light is focussed on to the photoreceptor layer of the retina at 4 dpf.

Paper Details

Date Published: 10 March 2015
PDF: 7 pages
Proc. SPIE 9334, Optical Methods in Developmental Biology III, 933408 (10 March 2015); doi: 10.1117/12.2079887
Show Author Affiliations
Laura K. Young, Durham Univ. (United Kingdom)
Miguel Jarrin, Durham Univ. (United Kingdom)
Christopher D. Saunter, Durham Univ. (United Kingdom)
Roy Quinlan, Durham Univ. (United Kingdom)
John M. Girkin, Durham Univ. (United Kingdom)

Published in SPIE Proceedings Vol. 9334:
Optical Methods in Developmental Biology III
Andrew M. Rollins; Scott E. Fraser; Michael A. Choma M.D., Editor(s)

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