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Proceedings Paper

In vivo pump-probe microscopy of melanoma: characterizing shifts in excited state photodynamics with respect to invasiveness
Author(s): Jesse W. Wilson; Simone Degan; Christina S. Gainey; Sanghamitra Deb; Christopher P. Dall; Yasmine Tameze-Rivas; Jennifer Zhang; Warren S. Warren
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Paper Abstract

Pump-probe microscopy is a multiphoton technique that generates molecular contrast from absorptive pigments, such as melanin. It holds the potential to be used as a non-invasive screening tool to discern whether a given early-stage melanoma has acquired the capacity for metastasis. Here, we examined lesions in a Braf(V600E)-driven model of melanoma to assess whether loss of the tumor suppressor gene Pten in a is accompanied by a shift in pigment expression, as measured in vivo by pump-probe microscopy. The data were analyzed to determine differences in the excited-state lifetime of melanins expressed in Pten-competent and Pten-loss pigmented lesions. Loss of the tumor suppressor Pten was found to be accompanied by a statistically significant decrease in pixel-average excited state lifetime (p = 1.3e-4).

Paper Details

Date Published: 5 March 2015
PDF: 5 pages
Proc. SPIE 9329, Multiphoton Microscopy in the Biomedical Sciences XV, 93290P (5 March 2015); doi: 10.1117/12.2079886
Show Author Affiliations
Jesse W. Wilson, Duke Univ. (United States)
Simone Degan, Duke Univ. (United States)
Duke Univ. Medical Ctr. (United States)
Christina S. Gainey, Duke Univ. (United States)
Sanghamitra Deb, Duke Univ. (United States)
Christopher P. Dall, Duke Univ. (United States)
Yasmine Tameze-Rivas, Duke Univ. Medical Ctr. (United States)
Jennifer Zhang, Duke Univ. Medical Ctr. (United States)
Warren S. Warren, Duke Univ. (United States)
Duke Univ. Medical Ctr. (United States)

Published in SPIE Proceedings Vol. 9329:
Multiphoton Microscopy in the Biomedical Sciences XV
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

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