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Proceedings Paper

In-vivo validation of fluorescence lifetime imaging (FLIm) of coronary arteries in swine
Author(s): Julien Bec; Dinglong Ma; Diego R. Yankelevich; Dimitris S. Gorpas; William T. Ferrier D.V.M.; Jeffrey Southard M.D.; Laura Marcu
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Paper Abstract

We report a scanning imaging system that enables high speed multispectral fluorescence lifetime imaging (FLIm) of coronary arteries. This system combines a custom low profile (3 Fr) imaging catheter using a 200 μm core side viewing UV-grade silica fiber optic, an acquisition system able to measure fluorescence decays over four spectral bands at 20 kHz and a fast data analysis and display module. In vivo use of the system has been optimized, with particular emphasis on clearing blood from the optical pathway. A short acquisition time (5 seconds for a 20 mm long coronary segment) enabled data acquisition during a bolus saline solution injection through the 7 Fr catheter guide. The injection parameters were precisely controlled using a power injector and optimized to provide good image quality while limiting the bolus injection duration and volume (12 cc/s, 80 cc total volume). The ability of the system to acquire data in vivo was validated in healthy swine by imaging different sections of the left anterior descending (LAD) coronary. A stent coated with fluorescent markers was placed in the LAD and imaged, demonstrating the ability of the system to discriminate in vivo different fluorescent features and structures from the vessel background fluorescence using spectral and lifetime information. Intensity en face images over the four bands of the instrument were available within seconds whereas lifetime images were computed in 2 minutes, providing efficient feedback during the procedure. This successful demonstration of FLIm in coronaries enables future study of atherosclerotic cardiovascular diseases.

Paper Details

Date Published: 26 February 2015
PDF: 5 pages
Proc. SPIE 9303, Photonic Therapeutics and Diagnostics XI, 93033M (26 February 2015); doi: 10.1117/12.2079833
Show Author Affiliations
Julien Bec, Univ. of California, Davis (United States)
Dinglong Ma, Univ. of California, Davis (United States)
Diego R. Yankelevich, Univ. of California, Davis (United States)
Dimitris S. Gorpas, Univ. of California, Davis (United States)
William T. Ferrier D.V.M., Univ. of California, Davis (United States)
Jeffrey Southard M.D., Univ. of California, Davis (United States)
Laura Marcu, Univ. of California, Davis (United States)

Published in SPIE Proceedings Vol. 9303:
Photonic Therapeutics and Diagnostics XI
Hyun Wook Kang; Brian J. F. Wong M.D.; Melissa C. Skala; Bernard Choi; Guillermo J. Tearney M.D.; Andreas Mandelis; Nikiforos Kollias; Kenton W. Gregory M.D.; Mark W. Dewhirst D.V.M.; Justus F. Ilgner M.D.; Alfred Nuttal; Haishan Zeng; Laura Marcu; Claus-Peter Richter, Editor(s)

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