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Proceedings Paper

H4 tail interactions revealed by fluorescent fluctuation spectroscopy
Author(s): Nathan Nurse; Chongli Yuan
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Paper Abstract

Post-translational modifications to histone tails nave been shown to play a large role in dictating the conformation of chromatin. Structural changes in chromatin can play a large role in gene expression as compact chromatin can occlude transcriptional machinery. The role of the flexible regions of H4 N terminal tails is investigated using fluorescent correlation spectroscopy and the photon counting histogram. The combination of these techniques allows for the distinction between intra-array and inter-array interactions, as well as reveals structural changes that result from these interactions. The H4 tail was found to partake in attractive intra-array interactions that compact the 6x167 nucleosome arrays but did not partake in inter-array interactions that lead to oligomerization.

Paper Details

Date Published: 9 March 2015
PDF: 9 pages
Proc. SPIE 9331, Single Molecule Spectroscopy and Superresolution Imaging VIII, 933104 (9 March 2015); doi: 10.1117/12.2079810
Show Author Affiliations
Nathan Nurse, Purdue Univ. (United States)
Chongli Yuan, Purdue Univ. (United States)

Published in SPIE Proceedings Vol. 9331:
Single Molecule Spectroscopy and Superresolution Imaging VIII
Jörg Enderlein; Ingo Gregor; Zygmunt Karol Gryczynski; Rainer Erdmann; Felix Koberling, Editor(s)

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