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Proceedings Paper

Low-intensity infrared laser effects on zymosan-induced articular inflammatory response
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Paper Abstract

Low-level therapy laser is a phototherapy treatment that involves the application of low power light in the red or infrared wavelengths in various diseases such as arthritis. In this work, we investigated whether low-intensity infrared laser therapy could cause death by caspase-6 apoptosis or DNA damage pathways in cartilage cells after zymosaninduced articular inflammatory process. Inflammatory process was induced in C57BL/6 mouse by intra-articular injection of zymosan into rear tibio-tarsal joints. Thirty animals were divided in five groups: (I) control, (II) laser, (III) zymosan-induced, (IV) zymosan-induced + laser and (V). Laser exposure was performed after zymosan administration with low-intensity infrared laser (830 nm), power 10 mW, fluence 3.0 J/cm2 at continuous mode emission, in five doses. Twenty-four hours after last irradiation, the animals were sacrificed and the right joints fixed and demineralized. Morphological analysis was observed by hematoxylin and eosin stain, pro-apoptotic (caspase-6) was analyzed by immunocytochemistry and DNA fragmentation was performed by TUNEL assay in articular cartilage cells. Inflammatory process was observed in connective tissue near to articular cartilage, in IV and V groups, indicating zymosan effect. This process was decreased in both groups after laser treatment and dexamethasone. Although groups III and IV presented higher caspase-6 and DNA fragmentation percentages, statistical differences were not observed when compared to groups I and II. Our results suggest that therapies based on low-intensity infrared lasers could reduce inflammatory process and could not cause death by caspase-6 apoptosis or DNA damage pathways in cartilage cells after zymosan-induced articular inflammatory process.

Paper Details

Date Published: 5 March 2015
PDF: 7 pages
Proc. SPIE 9321, Optical Interactions with Tissue and Cells XXVI, 932114 (5 March 2015); doi: 10.1117/12.2079785
Show Author Affiliations
Lúcia Mara Januário dos Anjos, Univ. Federal de Juiz de Fora (Brazil)
Adenilson de Souza da Fonseca, Univ. do Estado do Rio de Janeiro (Brazil)
Jacy Gameiro, Univ. Federal de Juiz de Fora (Brazil)
Flávia de Paoli, Univ. Federal de Juiz de Fora (Brazil)

Published in SPIE Proceedings Vol. 9321:
Optical Interactions with Tissue and Cells XXVI
E. Duco Jansen, Editor(s)

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