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Proceedings Paper

Penetration of spherical and rod-like gold nanoparticles into intact and barrier-disrupted human skin
Author(s): Christina Graf; Daniel Nordmeyer; Sebastian Ahlberg; Jörg Raabe; Annika Vogt; Jürgen Lademann; Fiorenza Rancan; Eckart Rühl
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Paper Abstract

The penetration of spherical and rod-like gold nanoparticles into human skin is reported. Several skin preparation techniques are applied, including cryo techniques, such as plunge freezing and freeze drying, and the use of wet cells. Their advantages and drawbacks for observing nanoparticle uptake are discussed. Independent of the particle shape no uptake into intact skin is observed by a combination of imaging approaches, including scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDX), and scanning X-ray microscopy (STXM). These results are discussed along with suitable skin preparation approaches. Experiments on barrier-disrupted skin, i.e. mechanical lesions made by pricking, indicate, however, that gold particles can be identified deep in the dermis, as follows from STXM studies on wet skin samples.

Paper Details

Date Published: 12 March 2015
PDF: 8 pages
Proc. SPIE 9338, Colloidal Nanoparticles for Biomedical Applications X, 93381L (12 March 2015); doi: 10.1117/12.2079719
Show Author Affiliations
Christina Graf, Freie Univ. Berlin (Germany)
Daniel Nordmeyer, Charité Universitätsmedizin Berlin (Germany)
Sebastian Ahlberg, Charité Universitätsmedizin Berlin (Germany)
Jörg Raabe, Swiss Light Source (Switzerland)
Annika Vogt, Charité Universitätsmedizin Berlin (Germany)
Jürgen Lademann, Charité Universitätsmedizin Berlin (Germany)
Fiorenza Rancan, Charité Universitätsmedizin Berlin (Germany)
Eckart Rühl, Freie Univ. Berlin (Germany)

Published in SPIE Proceedings Vol. 9338:
Colloidal Nanoparticles for Biomedical Applications X
Wolfgang J. Parak; Marek Osinski; Xing-Jie Liang, Editor(s)

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