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Proceedings Paper

Laser cross-linking protein captures for living cells on a biochip
Author(s): Chih-Lang Lin; Ming-Jeng Pan; Hai-Wen Chen; Che-Kuan Lin; Chuen-Fu Lin; Patrice L. Baldeck
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Paper Abstract

In this study, bio-sensing pads are proposed to capture living cells, which are fabricated on cover glasses by cross-linking proteins/antibodies using laser induced photochemistry. The biological functions of the cross-linked protein/antibody were verified by capturing Staphylococcus aureus (S. aureus), Leptospira, and red blood cells (RBCs), separately, with associated protein/antibody sensing pads. The experimental results show that S. aureus were bound on GFP-AcmA’ pad after minutes of incubation and phosphate buffered saline (PBS) rinsing. No binding was observed with reference pad made of neutral bovine serum albumin (BSA). Second, A-type RBCs were chosen as the model cell to demonstrate the blood typing feasibility of the anti-A pad in microchannel. The A-type RBCs were captured only by the anti-A pad, but not the reference pad made of BSA. The same experimental model was carried out on the Leptospira, which stuck on the blood serum pad after PBS rinsing, but not BSA pad. This study provides a potential platform for simple and direct detection of living full cells without culture that could be used in point-of-care settings.

Paper Details

Date Published: 2 March 2015
PDF: 6 pages
Proc. SPIE 9310, Frontiers in Biological Detection: From Nanosensors to Systems VII, 93100D (2 March 2015); doi: 10.1117/12.2079717
Show Author Affiliations
Chih-Lang Lin, Central Taiwan Univ. of Science and Technology (Taiwan)
Ming-Jeng Pan, Central Taiwan Univ. of Science and Technology (Taiwan)
Hai-Wen Chen, Central Taiwan Univ. of Science and Technology (Taiwan)
Che-Kuan Lin, Feng Chia Univ. (Taiwan)
Chuen-Fu Lin, National Chiayi Univ. (Taiwan)
Patrice L. Baldeck, Univ. Grenoble 1, CNRS, LIPhy (France)

Published in SPIE Proceedings Vol. 9310:
Frontiers in Biological Detection: From Nanosensors to Systems VII
Benjamin L. Miller; Philippe M. Fauchet; Brian T. Cunningham, Editor(s)

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