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Proceedings Paper

Optimizing experimental conditions for stimulated emission depletion microscopy in biophotonics
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Paper Abstract

Using a novel numerical method we show how to optimize the resolution enhancement of stimulated emission depletion (STED) by simulating the entire process including the absorption, overlapping multiple beams and stimulated emission. We provide calculations showing that for fixed donut pulse energy, a longer donut pulse length can result in greater resolution enhancement than a shorter donut pulse length. These results show how it is possible to use our simulations to design the best experimental conditions for STED resolution enhancement and illustrate the importance of having a software program that includes both multiple beams and stimulated emission.

Paper Details

Date Published: 13 March 2015
PDF: 7 pages
Proc. SPIE 9369, Photonic Instrumentation Engineering II, 93690V (13 March 2015); doi: 10.1117/12.2079479
Show Author Affiliations
Karl Beeson, Simphotek Inc. (United States)
Mary J. Potasek, Simphotek Inc. (United States)
Evgueni Parilov, Simphotek Inc. (United States)

Published in SPIE Proceedings Vol. 9369:
Photonic Instrumentation Engineering II
Yakov G. Soskind; Craig Olson, Editor(s)

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