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Proceedings Paper

Nonlinear imaging of lipid membrane alterations elicited by nanosecond pulsed electric fields
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Paper Abstract

Second Harmonic Generation (SHG) imaging is a useful tool for examining the structure of interfaces between bulk materials. Recently, this technique was applied to detecting subtle perturbations in the structure of cellular membranes following nanosecond pulsed electric field (nsPEF) exposure. Monitoring the cell’s outer membrane as it is exposed to nsPEF via SHG has demonstrated that nanoporation is likely the root cause for size-specific, increased cytoplasmic membrane permeabilization. It is theorized that the area of the membrane covered by these pores is tied to pulse intensity or duration. The extent of this effect along the cell’s surface, however, has never been measured due to its temporal brevity and minute pore size. By enhancing the SHG technique developed and elucidated previously, we are able to obtain this information. Further, we vary the pulse width and amplitude of the applied stimulus to explore the mechanical changes of the membrane at various sites around the cell. By using this unique SHG imaging technique to directly visualize the change in order of phospholipids within the membrane, we are able to better understand the complex response of living cells to electric pulses.

Paper Details

Date Published: 12 March 2015
PDF: 5 pages
Proc. SPIE 9326, Energy-based Treatment of Tissue and Assessment VIII, 93260T (12 March 2015); doi: 10.1117/12.2079452
Show Author Affiliations
Erick K. Moen, The Univ. of Southern California (United States)
Hope T. Beier, Air Force Research Lab. (United States)
Gary L. Thompson, Oak Ridge Institute for Science and Education (United States)
Andrea M. Armani, The Univ. of Southern California (United States)
Bennett L. Ibey, Air Force Research Lab. (United States)

Published in SPIE Proceedings Vol. 9326:
Energy-based Treatment of Tissue and Assessment VIII
Thomas P. Ryan, Editor(s)

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