We present an ultrahigh resolution dual modality optical resolution photoacoustic microsopy (OR-PAM) and spectral domain optical coherence microscopy (SD-OCM) system. The ultrahigh sub-micron lateral resolution is provided by the high numerical aperture of the objective lens used while the ultrahigh axial resolution is provided by the broadband OCT laser that covers 107 nm with a central wavelength of 840 nm. The synchronized simultaneous acquisition for the two modalities is achieved using a 40MHz FPGA. 2D-scanning is realized by two orthogonal translation stages (PI, 400 nm resolution). The transversal resolution of the system is 0.5 μm, the axial resolutions are 30 μm (PAM) and 4 μm (OCM), respectively. The values have been determined experimentally using nanospheres (diameter 10-200nm). For a demonstration of the imaging capability we present images from thin slices of different biological samples as well as in vivo imaging in the zebrafish embryo.

Date Published: 11 March 2015
PDF: 5 pages
Proc. SPIE 9323, Photons Plus Ultrasound: Imaging and Sensing 2015, 93232N (11 March 2015); doi: 10.1117/12.2079232

Published in SPIE Proceedings Vol. 9323:
Photons Plus Ultrasound: Imaging and Sensing 2015
Alexander A. Oraevsky; Lihong V. Wang, Editor(s)