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Proceedings Paper

Two-photon excited fluorescence microendoscopic imaging using a GRIN lens
Author(s): Wei Yan; Xiao Peng; Danying Lin; Qi Wang; Jian Gao; Jie Zhou; Tong Ye; Junle Qu; Hanben Niu
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Paper Abstract

With the rapid development of life sciences, there is an increasing demand for intravital fluorescence imaging of small animals. However, large dimensions and limited working distances of objective lenses in traditional fluorescence microscopes have limited the imaging applications mostly to superficial tissues. To overcome this disadvantage, researchers have developed the graded-index (GRIN) probes with small diameters for imaging internal organs of small animals in a minimally invasive fashion. Here, we present the development of a fluorescence endoscopic imaging system based on a GRIN lens using two-photon excitation. Experimental results showed that this system could perform dynamic fluorescence microendoscopic imaging and monitor the blood flow in anesthetized living mice using two-photon excitation.

Paper Details

Date Published: 5 March 2015
PDF: 4 pages
Proc. SPIE 9329, Multiphoton Microscopy in the Biomedical Sciences XV, 93290N (5 March 2015); doi: 10.1117/12.2079183
Show Author Affiliations
Wei Yan, Shenzhen Univ. (China)
Clemson Univ. (United States)
Xiao Peng, Shenzhen Univ. (China)
Danying Lin, Shenzhen Univ. (China)
Qi Wang, Shenzhen Univ. (China)
Jian Gao, Shenzhen Univ. (China)
Jie Zhou, Shenzhen Univ. (China)
Tong Ye, Clemson Univ. (United States)
Junle Qu, Shenzhen Univ. (China)
Hanben Niu, Shenzhen Univ. (China)

Published in SPIE Proceedings Vol. 9329:
Multiphoton Microscopy in the Biomedical Sciences XV
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

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