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Proceedings Paper

Multiphoton light-sheet microscopy using wavelength mixing: fast multicolor imaging of the beating Zebrafish heart with low photobleaching
Author(s): Pierre Mahou; Julien Vermot; Emmanuel Beaurepaire; Willy Supatto
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Paper Abstract

Two-photon laser scanning microscopy has become a standard to map thick and live tissues. However, its application for fast and multicolor imaging remains challenging. To address this issue, we report on the implementation of mixed wavelength excitation in a two-photon light-sheet microscope. We illustrate the potential of the technique by recording sustained multicolor two-photon movies of the beating heart in zebrafish embryos with negligible photobleaching at 28 million pixels/second. In particular, 3D reconstructions of the heart periodic motion are obtained with sufficient spatiotemporal resolution to track the fast movements of individual cells during a cardiac cycle.

Paper Details

Date Published: 5 March 2015
PDF: 7 pages
Proc. SPIE 9329, Multiphoton Microscopy in the Biomedical Sciences XV, 93290Z (5 March 2015); doi: 10.1117/12.2079176
Show Author Affiliations
Pierre Mahou, Lab. d’Optique et Biosciences, Ecole Polytechnique (France)
Julien Vermot, Institute of Genetics and Molecular and Cellular Biology, Univ. de Strasbourg (France)
Emmanuel Beaurepaire, Lab. d'Optique et Biosciences, Ecole Polytechnique (France)
Willy Supatto, Lab. d’Optique et Biosciences, Ecole Polytechnique (France)

Published in SPIE Proceedings Vol. 9329:
Multiphoton Microscopy in the Biomedical Sciences XV
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

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