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Proceedings Paper

Photodynamic therapy: a synergy between light and colors
Author(s): Elisabetta Merigo; Michele Sozzi; Tecla Ciociola; Stefania Conti; Carlo Fornaini M.D.; Paolo Vescovi; Stefano Selleri; Annamaria Cucinotta
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Paper Abstract

In this work the application of different laser wavelengths, in combination with different photosensitizing dyes, to bacterial cultures, in liquid or solid mean, has been investigated. Two types of Streptococcus mutans cultures have been used for the experiments, inside agar and saline solution. Three different laser wavelengths have been applied to the bacterial cultures together with a photosensitizing dye: red diode (650 nm) on cultures stained with Toluidine Blue, blueviolet diode (450 nm) on cultures stained with Curcumin and KTP laser (532 nm) on cultures stained with Erythrosine. The choice of the dye has been made considering the color affinity with the used wavelength. Tests without dyes have also been performed. Experimental results show that the maximum inhibition of bacterial growth with the blue laser has been obtained in a saline solution with a growth of 40.77%. While the combination with Curcumin lead to an inhibition growth of about 99.1%, for a laser fluence of 30J/cm2. No inhibition has been observed using the red laser in saline solution without dye, while the combination with Toluidine Blue resulted in a 100% inhibition growth for 20 and 30 J/cm2 fluences. An inhibition growth of just 16.26% has been obtained with the use of KTP laser in saline solution without dye. The use of Erythrosine had the effect of a complete inhibition growth. From the obtained results it is possible to observe that the combination of laser wavelength with a particular photosensitizing dye can dramatically increase the bacterial growth.

Paper Details

Date Published: 24 February 2015
PDF: 8 pages
Proc. SPIE 9306, Lasers in Dentistry XXI, 93060A (24 February 2015); doi: 10.1117/12.2079131
Show Author Affiliations
Elisabetta Merigo, Univ. degli Studi di Parma (Italy)
Michele Sozzi, Univ. degli Studi di Parma (Italy)
Tecla Ciociola, Univ. degli Studi di Parma (Italy)
Stefania Conti, Univ. degli Studi di Parma (Italy)
Carlo Fornaini M.D., Univ. degli Studi di Parma (Italy)
Paolo Vescovi, Univ. degli Studi di Parma (Italy)
Stefano Selleri, Univ. degli Studi di Parma (Italy)
Annamaria Cucinotta, Univ. degli Studi di Parma (Italy)

Published in SPIE Proceedings Vol. 9306:
Lasers in Dentistry XXI
Peter Rechmann; Daniel Fried, Editor(s)

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