Second-harmonic-generation (SHG) microscopy is a new tool for observing the collagen fiber in tissue in vivo. Conventional SHG microscopy equipped with 100-fs pulse laser is insufficient to visualize low-order-structured, immature collagen with high contrast (for example, collagen fiber produced by cultured cell) because of low nonlinear susceptibility χ⁽²⁾. To enhance the image contrast while avoiding the sample damage, one should increase a peak power of the laser light while maintaining the same average power as the 100-fs laser. In this paper, we constructed sensitive SHG microscopy equipped with a 10-fs Ti:Sapphire laser and succeeded to visualize collagen fibers produced by the cultured osteoblasts in vivo.

Date Published: 5 March 2015
PDF: 6 pages
Proc. SPIE 9329, Multiphoton Microscopy in the Biomedical Sciences XV, 93292Q (5 March 2015); doi: 10.1117/12.2079114

Published in SPIE Proceedings Vol. 9329:
Multiphoton Microscopy in the Biomedical Sciences XV
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)